You can lock and unlock folder by using Command Prompt (CMD).
Open Command Prompt (CMD) with administrator rights. Type below command for lock folder.
C:\Windows\system32>cacls <FolderPath> /E /P everyone:n
You can check whether the folder is accessible or not by simply clicking on the locked folder. If deny message pop up comes, indicates folder is locked.
You can unlock folder by type below command in CMD.
C:\Windows\system32>cacls <FolderPath> /E /P everyone:f
Now, you can access the folder by simply clicking it.
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Disclaimer: This tutorial is for educational purpose only. Individual is solely responsible for any illegal act.